周期性机械牵拉通过上调Runx2表达促进MC3T3-E1细胞迁移
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1.上海市第六人民医院;2.上海交通大学

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Cyclic Stretch Promotes Migration of MC3T3-E1 Cells via Increasing Runx2 Expression
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    摘要:

    目的 探究周期性牵拉对小鼠胚胎成骨细胞(MC3T3-E1)迁移功能的影响及Runx2在该过程中发挥的作用。方法 使用FLEXCELL-5000 型应变加载系统对体外培养的MC3T3-E1细胞施加15%幅度的牵拉,模拟细胞体内受力情况,使用划痕愈合实验表征MC3T3-E1细胞的迁移功能,使用蛋白免疫印迹法检测Runx2的表达情况,使用RNA干扰技术特异性降低Runx2的表达量。结果 幅度为15%的周期性机械牵拉可以促进MC3T3-E1细胞的迁移。干扰Runx2能抑制MC3T3-E1细胞的迁移,部分降低机械牵拉促进细胞迁移的效应。结论 周期性牵拉可以促进MC3T3-E1细胞的迁移,在此过程中Runx2可能发挥重要作用。本研究探究了机械牵拉促进MC3T3-E1细胞迁移的相关机制,旨在为寻找促进骨折愈合的创新临床治疗方法提供实验依据。

    Abstract:

    Objective To investigate the effect of cyclic stretching on migration of MC3T3-E1 cells and related mechanism. Methods The strain loading system Flexcell-5000 was used to stretch MC3T3-E1 cells with 15% amplitude to simulate the mechanical condition in vivo. The wound healing assay was used to measure the migration of MC3T3-E1 cells. Western blotting was used to showed Runx2 expression. RNA interfering was used to decrease Runx2 expression. Results Cyclic stretching boosts migration of MC3T3-E1 cells. Decrease Runx2 expression can inhibit the migration of MC3T3-E1 cells and partially offset the effect of mechanical stretch on cell migration. Conclusion Cyclic stretch can promote the migration of MC3T3-E1 cells, and Runx2 may play an important role in this process. This study explored the mechanism of mechanical stretching promoting the migration of MC3T3-E1 cells, in order to provide experimental basis for finding innovative clinical treatment methods to promote fracture healing.

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  • 收稿日期:2021-03-18
  • 最后修改日期:2021-05-09
  • 录用日期:2021-05-11
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