University of Shanghai for Science and Technology
目的：在射频能量的作用下，通过新型压力可控电极对猪小肠(回肠部分)进行焊接，验证能量组织焊接技术对于肠道结构重建的可行性和安全性。方法：将新鲜猪小肠按“粘膜-浆膜”嵌套在负电极上，通过施压圆锥体对正电极施加不同的压合压强（497，796，995，1194，1492 kPa），在射频能量的作用下完成肠道组织的焊接，通过撕脱力和爆破压测试研究焊接吻合口的生物力学特性，并对组织热扩散和微观组织结构进行检查。结果：在能量输出功率160 W，压合压强995 kPa，焊接时间为13s时，肠道吻合口呈现最优的生物力学特性，其撕脱力和爆破压分别达到8.73±1.11N和62.2±3.08 mmHg，且组织微观结构较完整，并能观察到少量游离胶原蛋白。结论：射频能量组织焊接技术具有良好的应用前景，能够实现肠道组织快速、稳定的连接，对缩短手术时间、简化操作流程并提高手术质量具有重要意义。
Objective: We fused the ileum of porcine intestines with radiofrequency (RF) energy through a novel linkage-type pressure controlled electrode with the aim of verifying the feasibility and security of the RF energy tissue fusion technology. Methods: Fresh porcine intestines were fixed on the negative electrode in the order of "mucosa-serosa", and then different compressive pressures（497，796，995，1194，1492 kPa）and RF energy were applied to the tissue through positive electrode to complete anastomosis. Biomechanical properties of fused area were studied by tensile strength and bursting pressure test. At last, the thermal diffusion and tissue microstructure were studied to evaluate the effect of anastomosis. Results: The anastomotic tensile strength and bursting pressure can reach 8.73±1.11N and 62.2±3.08 mmHg respectively when the energy output power, pressure and welding time are 160 W, 995 kPa and 13s, respectively, and an intact microstructure with little free collagen in the fusion area can be observed. Conclusion: The technology of tissue fusion based on radiofrequency energy could accomplish fast and stable intestinal tract reconstruction, which shows great potential in clinical application. It is of great significance to shorten the operation time, simplify the operation process and improve the operation quality.