国家自然科学基金面上项目(81871827),深圳市基础研究项目 (GJHZ20170314154914747,JCYJ20170818155915362 和 GJHS20170314153933234)
Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences
摘要: 目的 探究封闭性水凝胶力学性质与其止血性能之间的关系。 方法 以透明质酸/明胶水凝胶为例，测量其凝固时间、弹性模量、粘性模量、顶破强度和止血性能，探讨水凝胶性质与其止血性能的关系，与医用纤维蛋白胶止血剂对比，提出凝固时间和力学参数，用于判断封闭性水凝胶应用于临床止血的可行性。 结果 凝固时间较长、弹性模量、粘性模量和顶破强度较小的水凝胶只能对小出血量起控制作用，凝固时间短、弹性模量、粘性模量和顶破强度大的水凝胶能有效减少大出血量情况下的血液损失。 结论 水凝胶的凝固时间、弹性模量、粘性模量以及顶破强度与其止血性能有关，如果水凝胶封闭止血要达到纤维蛋白胶的止血水平，其凝固时间不要高于120 s，弹性模量不要低于600 Pa，粘性模量不要低于120 Pa，对于直径2mm的组织模型破损，顶破强度不要低于10.7 kPa，最好高于16.0 kPa。 关键词: 可注射水凝胶；流变性质；顶破强度；止血
Abstract: Objective The aim of this study was to investigate the correlation between the mechanical properties and hemostatic ability of the sealing hydrogels. Method Taking hyaluronic acid/gelatin hydrogels as the examples, the gelation time, elastic modulus, viscous modulus, bursting strength and hemostatic ability of the hydrogels were measured to investigate the correlation between mechanical properties and hemostatic ability of sealing hydrogels. Compared with medical hemostatic agent, fibrin glue, the gelation time and mechanical parameters were proposed to judge the feasibility of sealing hydrogels to be used for hemostasis in clinic. Results Hydrogels with a long gelation time, low elastic modulus, low viscous modulus and small bursting strength were merely suitable for hemostasis in minor bleeding. The hydrogels with a short gelation time, high elastic modulus, high viscous modulus and large bursting strength could effectively reduce the blood loss in the case of massive bleeding. Conclusions The hemostatic ability of a hydrogel is correlated to its gelation time, elastic modulus, viscous modulus and bursting strength. To achieve hemostasis as effective as fibrin glue, a sealing hydrogel should gel less than 120 s, its elastic modulus and viscous modulus should exceed 600 Pa and 120 Pa, and its burst strength should not be less than 10.7 kPa, preferably larger than 16.0 kPa, for the tissue model with the damage diameter in 2 mm.